In vitro Human Tissue Development
Prellis uses holographic laser technology to print biomimetic vascular channels that support the development of human tissues.
Prellis human tissues are engineered using our ultrafast, high-resolution 3D printing technology capable of fabricating biomimetic tissue scaffolds with architectural and structural complexity at the micron scale. These tissue engineered scaffolds are designed to efficiently deliver oxygen and nutrients to densely packed cell clusters, eliminating the requirement for spontaneous organoid formation, and can be used as complex co-culture platforms for various therapeutic, disease modeling, and regenerative medicine applications.
Holographic Laser Printing
Prellis’ proprietary holographic laser printing technology enables rapid construction of high-resolution biocompatible scaffolds.
Prellis bioprints complex micron-feature sized scaffolds up to 350 times faster than other laser printing technologies. Prellis holographic printing is fully integrated with internally developed software, chemistry, and optical engineering technology.
High Resolution Bioprinting Enables Access to Human Biology
Tissue scaffolds are printed in various conformations with tunable biochemical and mechanical properties. Prellis tissue models can be seeded with multiple cell types sequentially, allowing for complex co-cultures.
HUVECs grown on Prellis scaffolds for 5 days
A critical challenge in printing biomimetic tissues is replicating the native tissue structural and architectural complexity that supports cell growth. Ultra fine resolution allows for printing vascular-like channels and microarchitecture that mimic human biology. The thin, gas and nutrient permeable channel walls of the microstructures and vessels facilitate nutrient diffusion in a cell-dense environment.
Scaffolds support complex microfluidics, cell adhesion, and growth.
Prellis' Hyperdrive CAD file, cross-section, and scaffold
Large 3D Tissues in vitro
Large organoids and tissue models can be grown in vitro in flow-free culture, reducing the time to organoid development while maintaining the properties of a 3D organoid. Organoid scaffolds are laser printed using transplantable hydrogel material allowing for both in vitro studies and transplantation of the same tissues into animal models. Proteins, growth factors, and small molecules can be incorporated into the scaffold to support cell adhesion, growth, and differentiation.
Applications for 3D Tissue Models
The ability to precisely engineer scaffold architecture bypasses the need to rely on spontaneous organoid formation or in vitro vascularization, supporting large tissue growth.
Immune System (EXIS™)
Prellis Biologics has developed human lymph node organoids (LNOs™) that mimic the human immune response, in vitro, allowing for rapid fully human antibody discovery.
Ki67 DAPI Scaffold
MCF7 cell line grown on Prellis scaffold for 5 days
Tumor cells grown in 3D have distinct gene profiles that more closely mimic tumors found in vivo. Prellis tumor models are up to 2 millimeters in diameter and can be used in longitudinal treatment studies. Tumor organoids support co-culture of tumor cells (primary and cell lines), immune cells, and endothelial cells.
HCT116 and human APCs
HCT116 and immune cell co-culture at 72 hours
Larger non-hypoxic human tissue models can be grown from primary cells using Prellis scaffolds. Prellis has successfully grown large liver chips for over 30 days under perfusion.
Albumin CD31 Scaffold
3D confocal z-stack of 33 day human hepatocyte grown on Prellis’ 5 mm x 5 mm x 2 mm scaffold
Access to living human neural tissue for longitudinal therapeutic studies is extremely limited. Prellis is actively developing 3D neural tissue cultures that include microvasculature and microfluidic flow. These cell cultures can be imaged and maintained under standard laboratory conditions.