Organoid

Scaffolds

Guaranteed Spheroid Growth.

Organoids are biocompatible structures that hold your precious cells in place with an oxygen permeable 3D scaffold, guaranteeing your 3D tissue culture will work every time.

No more waiting for spheroid growth.

In less than 24 hours

Begin testing 3D cell responses

Transplant into an animal model

Induce cell differentiation in a 3D scaffold

Why screen therapeutics in 3D cell culture?

More accurate drug screening results
Differential cell gene expression relative to 2D
Better replicates the in vivo environment
Improved human to animal translation

    When you have complexity, like in [conventional] organoids or in animals – you increase variability. So we think we can reduce variability using the [Prellis] organoids especially going from the organoid to in vivo. The structures are also more consistent, so less variability from that perspective too. Less variability means quicker, with less cost and more tests – to results.

Marcus Muench, PhD
Associate Adjunct Professor
UCSF

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Small 3D tissues. Huge R&D potential. 

Primary Tumor 

Primary Skeletal

Cardiac Muscle

Cell Lines: HCT116, A549

Keratinocytes

Fibroblasts

Primary Immune Cells

Primary Fetal Liver

HUVECS

IPSCs

Glial Cells 

Astrocytes

use with over 15 primary cell types

Example applications

3D Cell Culture 

Animal Transplantation

Microfluidics Set Ups 

Long-term 3D Tissue Growth (4 wks)

Drug Screening 

Developmental Biology Studies 

Cell-Cell Interaction Studies

Multi-cellular Organism Studies

Confocal Microscopy

Two-Photon Imaging

Live/Dead Cell

FACS 

Western Blot

DNA/RNA Sequencing

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Tissue Sectioning 

compatible with a variety of assays

    The issue of vascularization of the organoid models is key. The traditional organoids are not healthy, because they have no vasculature, which is what gets nutrients through the structure. Really it is the tissue, just cells piled on to each other [and] in the center of that pile they have no nutrients, no oxygen – and they die. So the idea is that the Prellis structure, with the vasculature, keeps it from dying.

Jennifer Erwin, PhD
Assistant Professor
Johns Hopkins University

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Application Example: Animal Transplant

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Vascular organoid seeding

3 weeks post-tumor transplant

in vitro vascularization of organoid scaffold

Additional features:

Easy to handle

Simple set-up

96 well plate compatible 

No special flow systems or media necessary

Guaranteed organoid formation

Store organoids for up to 6 months

Seed over 200,000 cells in minutes

Can be coated with growth factors or matrix

© Prellis Biologics 2020, All rights reserved.