TISSUE ENGINEERING JOURNAL CLUB
We believe that bringing the entire tissue engineering community together could make a significant impact for everyone and we started taking the first steps towards achieving that. Email Greg@PrellisBio.com to find out more info about upcoming events!
Speaker: Olaia Vila, PhD Postdoctoral Researcher Gladstone Institute, UCSF
Talk Title: Engineering Neuromuscular Circuits
Dr. Vila shared her cutting edge research using neuromuscular junctions grown in 3D to examine changes in muscle control in myasthenia gravis.
Reference Publication: In vitro models of neuromuscular junctions and their potential for novel drug discovery and development Vila, O.F, et al. Expert Opinion on Drug Discovery March, 2020
Past Tissue Engineering Journal Club Events
Speaker: Iman Yazdi, PhD Principal Biomedical Engineer at LiquiGlide
Talk Title: Harnessing Biomaterials Potential for Matrix-Guided Angiogenesis
Dr. Yazdi shared a deep-dive on the usage of various biomaterials in the development of complex tissues, drug delivery and reviewed recent advances in biomedical engineering with live cells to induce angiogenesis.
Reference Publications: Synthetic nanoparticles functionalized with biomimetic leukocyte membranes possess cell-like functions. Nature Nanotechnology January 2013
Speaker: Gavan Wilhite VP of Software Engineering Prellis Biologics
Talk Title: A Software Approach: From Games to Tissue
Gavan Wilhite shares how he is bringing the principles of 3D game design to the development of complex physiologic human tissues.
Speaker: Aravind Natarajan, PhD Postdoctoral Research Fellow in Hematology at the Stanford University School of Medicine
Talk Title: Engineering E. coli to produce mucin-type O-glycoproteins
Reference Publication: A cell-free biosynthesis platform for modular construction of protein glycosylation pathways. Nature Communications, Kightlinger W. et al November, 2019.
Speaker: Melanie Matheu, PhD Founder and CEO of Prellis Biologics
Talk Title: A review of current bioprinting methods and recent tissue engineering advances
Dr. Matheu provided an overview of the status of the tissue engineering field by reviewing recent publications in the space.
Designing Biomimetic Triple-Layered Nanofibrous Vascular Grafts via Combinatorial Electrospinning Approach. J Nanosci Nanotechnol, Oct 1, 2020
Hair-bearing human skin generated entirely from pluripotent stem cells. Nature, June 3, 2020.
Harnessing nerve–muscle cell interactions for biomaterials‐based skeletal muscle regeneration. Society For Biomaterials, June 3, 2020
Speaker: Courtney Gegg, PhD Senior Director of Tissue Engineering
Talk Title: Engineering Cartilage with Biomimetic Structure and Function
Dr. Gegg reviewed methods and approaches for replicating the structure and function of natural cartilage for therapeutic applications.
Spatially patterned microribbon-based hydrogels induce zonally-organized cartilage regeneration by stem cells in 3D. Acta Biomater, Oct 9, 2019
Thyroid hormones enhance the biomechanical functionality of scaffold-free neocartilage. Arthritis Res Ther, February 11, 2015
Speaker: Milad Khorrami, PhD Director of Chemistry, Prellis Biologics
Talk Title: Fabrication of 3D Microstructures for Organic Bioelectronics and Tissue Engineering Applications
Dr. Khorrami outlined a practical approach to bioprinting electrical networks capable of supporting cell growth.
Reference publication: Conducting polymer microcontainers for biomedical applications. Annu Int Conf IEEE Eng Med Biol Soc, July 2017
Speaker: Priya Mohindra, PhD Postdoctoral Scholar, UCSF
Talk Title: Hang on Tight: Reprogramming the Cell with Microstructural Cues
Dr. Mohindra provided a detailed review of the role of materials in cell adhesion and development, a critical component of tissue engineering.
Reference publication: Injectable hyaluronic acid based microrods provide local micromechanical and biochemical cues to attenuate cardiac fibrosis after myocardial infarction. Biomaterials, March 24, 2018
Speaker 1: Sara R-Vaziri, PhD Postdoctoral Fellow, Stanford University
Talk Title: Heterogeneity and Genetic Variation of Cardiac Cells on cardiac Injury and Repair
Dr. R-Vaziri is a cardiac tissue development specialist who shared her cutting edge research in cardiac tissue development.
Reference publication: Park S, Ranjbarvaziri S, Lay FD, Zhao P, Miller MJ, Dhaliwal JS, Huertas-Vazquez A, Wu X, Qiao R, Soffer JM, Rau C, Wang Y, Mikkola HKA, Lusis AJ, Ardehali R. Genetic Regulation of Fibroblast Activation and Proliferation in Cardiac Fibrosis. Circulation. 2018 Sep 18;138(12):1224-1235. doi: 10.1161/CIRCULATIONAHA.118.035420. PMID: 29950403; PMCID: PMC6202226.
Speaker 2: Subhadeep Das, PhD Founder and CEO of Convalesce
Talk Title: Neural Tissue Engineering for Therapeutics
Dr. Das provided insight into the importance of novel biomaterials for therapeutic cell transplant in neuronal tissues.
Das, S., Zhou, K., Ghosh, D. et al. Implantable amyloid hydrogels for promoting stem cell differentiation to neurons. NPG Asia Mater 8, e304 (2016). https://doi.org/10.1038/am.2016.116
Das S, Jacob RS, Patel K, Singh N, Maji SK. Amyloid Fibrils: Versatile Biomaterials for Cell Adhesion and Tissue Engineering Applications. Biomacromolecules. 2018 Jun 11;19(6):1826-1839. doi: 10.1021/acs.biomac.8b00279. Epub 2018 May 9. PMID: 29701992. https://pubmed.ncbi.nlm.nih.gov/29701992/
Speaker: Mayasari Lim, PhD Regional Account Manager at RoosterBio
Talk Title: Radically Simplifying Clinical Translation and Scale Up of hMSCs for Tissue Engineering
Sourcing of cells for large scale tissue engineering is a critical roadblock when working to develop 3D tissues. Dr. Lim shared how RoosterBio
2. Angelopoulos, Ioannis & Allenby, Mark & Lim, Mayasari & Zamorano, Mauricio. (2019). Engineering inkjet bioprinting processes toward translational therapies. Biotechnology and Bioengineering. 117. 10.1002/bit.27176.
Speaker: Tomas Gonzalez-Fernandez, American Heart Association postdoctoral fellow in the Leach Laboratory at the University of California, Davis
Talk Title: Cell-Instructive Materials for 3D Bioprinting: From Stem Cells to Functional Musculoskeletal Tissues
Dr. Gonzalez-Fernandez’ research focuses on the combination of stem cells, biomaterials, and 3D bioprinting strategies for musculoskeletal tissue engineering. He received his PhD in Bioengineering from Trinity College Dublin. At Trinity College, he researched the development of novel bioinks for spatial gene delivery for cartilage and bone 3D printing.
Speaker: Dr. Stephanie Willerth, Chief Executive Officer at Axolotl Biosciences | Canada Research Chair and a full professor of Biomedical Engineering at the University of Victoria | Acting Director of the Centre for Biomedical Research.
Talk Title: 3D Bioprinting Complex Tissue Models
Dr. Stephanie Willerth’s research combines stem cells, tissue engineering, drug delivery, cellular reprogramming, and 3D bioprinting.
S2468451120300118 Walus, K., Beyer, S., and Willerth, S.M. 3D bioprinting healthy and disease models of brain tissue using stem cells. Current Opinion in Biomedical Engineering, 2020, 14. 25-33
2. Davoodi, E.,...W., Willerth, S.M., Extrusion and microfluidic- based bioprinting to fabricate biomimetic tissues and organs. Advanced Materials Technologies. 1901044.1-30.
Speaker: Theo Roth CTO of Arsenal Bio
Talk Title: Reprogramming Immune Cell Function and Specificity
Theo Roth, CTO of Arsenal Bio shared the team's cutting edge work and CRISPR-based genetic engineering of human T cells.
1. Nguyen, D.N., Roth, T.L., Li, P.J. et al. Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency. Nat Biotechnol 38, 44–49 (2020)
2. Leenay, R.T., Aghazadeh, A., Hiatt, J. et al. Large dataset enables prediction of repair after CRISPR–Cas9 editing in primary T cells. Nat Biotechnol 37, 1034–1037 (2019).
3. Simeonov DR, et al. A large CRISPR-induced bystander mutation causes immune dysregulation. Commun Biol. 2019 Feb 18;2:70.
Speaker: Mubhij Ahmad, MSc. Co-founder of DNALite Therapeutics
Talk Title: Using the GI tract as an Engineering Platform
Mubhij Ahmad shared the promise of a therapeutic delivery system that leverages the machinery of human cells. He discussed the development of human tissues for transplantation, clean meat, stem cell sourcing, and applications of associated technologies. He emphasized and further discussed how the GI tract he is looking at utilizing the GI tract as a means of an Engineering platform.
Reference publication: https://indiebio.co/dnalite-new-age-medicine-emerging/
Speaker 1: Hunter Jackson, PhD Computational Biology, 3Scan
Talk Title: Pre-Press: Mapping Neurons in the Cardiac System
Dr. Jackson shared a preprint article that defined the RNA expression patterns in cardiac neurons that defined the 3D spatial location of specific nerves in the rat heart.
Reference publication: Zimmer, T., Haufe, V., & Blechschmidt, S. (2014). Voltage-gated sodium channels in the mammalian heart. Global cardiology science & practice, 2014(4)
Speaker 2: Renaud Schuck, PhD Lead Engineer at Koniku
Talk Title: Discussing the use of Neurons in Detection Platforms
Dr. Schuck shared insights into the novel technology of in vitro neuron growth and using neurons as a detection platform for small molecules in the environment.
Reference publication: R Schuck, MA Go, S Garasto, S Reynolds, PL Dragotti, SR Schultz. Multiphoton minimal inertia scanning for fast acquisition of neural activity signals. Journal of neural engineering. 15 (2) (2018).
Speaker: Dr. Shubhankar Nath, Ph.D., Senior Scientist at CELLINK
Talk Title: 3D Bioprinted Models for Immuno-oncology Applications
Dr. Shubhankar Nath received his doctorate from The University of Texas at Austin in 2016. His thesis work focused on the secretory mechanisms of T cells, and his postdoctoral training was in photodynamic therapy at the Wellman Center for Photomedicine at Massachusetts General Hospital and Harvard Medical School under the mentorship of Professor Tayyaba Hasan. He is currently a Senior Scientist at CELLINK where he is developing 3D bioprinted tissue and tumor models for drug discovery and regenerative medicine.
Reference Publication: Kercher EM, Nath S, Rizvi I, Spring BQ. Cancer Cell-targeted and Activatable Photoimmunotherapy Spares T Cells in a 3D Coculture Model. Photochem Photobiol. 2020
Our next Tissue Engineering Journal Club:
March 9th, 1pm PST
Speaker: Dr. Callie Higgins, a Materials Research Engineer at the National Institute of Standards and Technology
Talk Title: Vat Photopolymerization: A Transformative Tool for Regenerative Medicine
Dr. Higgins’ presentation, Vat Photopolymerization: A Transformative Tool for Regenerative Medicine, focuses on supporting innovation in the photopolymer additive manufacturing industry by enabling unprecedented high-resolution, mechanically-precise vat photopolymerization via fundamental understanding informed by novel voxel and sub-voxel-scale characterization throughout all major stages of the printing process. This technology has the potential to transform tissue engineering via precise, micron-scale property control, which was the focus of her PhD research at the University of Colorado.
Speaker: Dr. Trivia Frazier, PhD, MBA is the CEO and President of Obatala Sciences, a biotechnology toolkit company based in New Orleans, LA.
Talk Title: Fat-on-a-Chip for Drug Discovery and Preclinical Studies
Dr. Frazier will discuss the company’s Obacell Fat-on-a-Chip™ System for Drug Discovery, which is an adipocyte organoid system consisting of ObaGel™, Obatala’s human-derived hydrogel, in-house isolated stem cells, and in-house manufactured adipogenic media. The ObaCell™ system can be used for drug efficacy studies targeting new treatments for diabetes, obesity, and cancer and can be used in co-cultures with other cells to create mutli-organ models.